Development of a real-time PCR-based method for rapid differential identification of Mycobacterium species
نویسندگان
چکیده
منابع مشابه
A rapid, early detection of oral squamous cell carcinoma: Real time PCR based detection of tetranectin
The current study is focused on determining the mRNA expression levels of tetranectin, to detect oral squamous cell carcinoma (OSCC) and thus aiding in its classification at an early stage. RNA was isolated and cDNA synthesis was performed from the saliva samples of the patients and healthy individuals. A semiquantitative PCR based analysis was performed prior to quantitative and expression bas...
متن کاملA rapid, early detection of oral squamous cell carcinoma: Real time PCR based detection of tetranectin
The current study is focused on determining the mRNA expression levels of tetranectin, to detect oral squamous cell carcinoma (OSCC) and thus aiding in its classification at an early stage. RNA was isolated and cDNA synthesis was performed from the saliva samples of the patients and healthy individuals. A semiquantitative PCR based analysis was performed prior to quantitative and expression bas...
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BACKGROUND Brachyspira species are fastidious anaerobic microorganisms, that infect the colon of various animals. The genus contains both important pathogens of livestock as well as commensals. Two species are known to infect humans: B. aalborgi and B. pilosicoli. There is some evidence suggesting that the veterinary pathogenic B. pilosicoli is a potential zoonotic agent, however, since diagnos...
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The rapid detection and identification of Candida species in clinical laboratories are extremely important for the management of patients with hematogenous candidosis. Currently available culture and biochemical methods for detection and identification of Candida species are time-consuming. This study describes the use of a simple and rapid PCR method using species-specific oligonucleotides for...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2007
ISSN: 0266-8254,1472-765X
DOI: 10.1111/j.1472-765x.2007.02278.x